The ratio of hydrophilic residues to total number of amino acids is reported in %.įor more information on the calculator, please read our details document. The hydrophilicity value for each amino acid in the peptide sequence is indicated in a bar graph. The calculation of the average hydrophilicity of a peptide is based on the data from Hopp&Woods. The isoelectric point is calculated by approximation (accuracy ± 0.01). The isoelectric point calculator provides the pH at which the peptide net charge is zero.
The pKa values for cysteine (pKa = 8.33), aspartic acid (pKa = 3.86), glutamic acid (pKa = 4.25), histidine (pKa = 6.0), lysine (pKa = 10.53), arginine (pKa = 12.48), tyrosine (pKa = 10.07), the N-terminal (pKa = 9.69) and C-terminal (pKa = 2.34) are based on the Principles of Biochemistry, Lehninger (1982).
PKaj, pKa: values of the C-terminus and the aspartic acid, glutamic acid, cysteine and tyrosine residues
Nj: Number of of aspartic acid, glutamic acid, cysteine, and tyrosine residues and the C-terminus PKai, pKa: values of the N-terminus and the arginine, lysine, and histidine residues Ni: Number of arginine, lysine, and histidine residues and the N-terminus The peptide net charge calculator at a given pH is based on the formula below:
#MASS FINDER USING MOLARITY CODE#
Mi: Molecular weight of the amino acid residuesįor the molecular weight amino acid calculator, you can enter the 1- or 3- letter code of the desired amino acid, and the tool will provide the value the same way it would calculate peptide molecular weight. M: Molecular weight of the amino acid sequence To calculate the different features of the peptide, the calculator will apply the formulas described below:įor the peptide molecular weight calculator and molecular weight amino acid calculator, the following formula is applied: pH and a hydrophobicity calculator displayed in a plot. With the calculator and its easy use, peptide chemists can have access to a molecular weight peptide calculator and amino acid calculator, the isoelectric point, a peptide net charge calculator at neutral pH, the average hydrophilicity, the percentage of hydrophilic amino acids, the plot of the net charge vs. In this case, the MW is found to be 200 g/L.The calculator is one of the most useful tool for the peptide chemist to calculate peptide molecular weight and more. Next, determine the molecular weight of the solution. This solution is measured to have a volume of 2 liters. Next, determine the volume of the solution. For this example problem, the mass is measured to be 500 grams. The following example problem outlines the steps and information needed to calculate concentration when given mass.įirst, determine the mass.
#MASS FINDER USING MOLARITY HOW TO#
How to calculate concentration from mass? Knowing all 3 of these variables allows for the computation of the concentration.Ĭoncentration is usually measured or calculated in units of grams per mole, denoted g/mol. Type the percentage value and the evaluated density will be. How is concentration calculated from mass?Ĭoncentration is related to mass through the molecular weight and the volume of the solution. Just select your substance from the list and its molecular mass will appear in the calculator.
The following formula is used to calculate a concentration from the mass. Enter the mass, volume, and molar weight into the calculator to determine the concentration.
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